The goal of this research was to guage the consequences of vitamin E related with rapid thawing on cryopreserved goat semen. Two bucks had been used and eight ejaculates per animal had been collected utilizing synthetic vagina.
Semen was diluted with the next therapies: BIOXCELL (management), BIOXCELL + Equex (sodium lauryl sulphate) and BIOXCELL + vitamin E 100 μM. Semen was packaged into 0.25 mL straws and cooled at 5°C for 1 hour. Freezing was carried out in liquid nitrogen vapor (-155°C) throughout 15 minutes. Then, the straws had been immersed in liquid nitrogen (-196°C).
Straws had been thawed at 38°C/60 seconds or at 60°C/7 seconds with instant sperm evaluation. Hypoosmotic swelling take a look at was carried out including a 20 μL aliquot of thawed semen to 1 mL of hypoosmotic answer (100 mOsm · Kg(-1)) adopted by incubation throughout 60 minutes in water bathtub (38°C). Vitamin E didn’t have an effect on any studied parameters (P>> 0.05).
Nevertheless, defrosting price of 60°C/7 seconds improved sperm membrane purposeful integrity (P < 0.05). Current data about goat semen cryopreservation isn’t ample to make sure excessive post-thawing restoration charges; thus, this research brings necessary knowledge about utilizing antioxidants and completely different thawing charges on cryopreservation course of.
Characterization and cryopreservation of semen from endangered markhor goats (Capra falconeri heptneri) with analysis of reproductive seasonality.
The goals of this research had been to find out the reproductive seasonality of 4 captive markhor goats (Capra falconeri heptneri), to characterize semen collected by electroejaculation, and to match extenders and processing methods for semen cryopreservation.
Over the course of 1 yr, imply month-to-month scrotal circumference, serum testosterone, and fecal testosterone had been measured and located to be inversely related with day size. Maximum scrotal circumference (25.2 +/- 0.9 cm), serum testosterone (521.0 +/- 103.four ng/dl), and fecal testosterone (382.5 +/- 90.
Three ng/g) occurred in November, when day size was brief (9.7 +/- 0.1 hr). Once a month for Three mo (December, January, and February), bucks had been anesthetized for electroejaculation and semen analysis. Semen samples had been divided into six aliquots for extension and cryopreservation in soy-based Bioxcell or Tris-based extender with 5 or 15% egg yolk, with and with out centrifugation.
Samples had been then thawed for repeat analysis 1-Three mo later. Postthaw analysis revealed no important variations between centrifuged and noncentrifuged samples.
Sperm in Tris 5% and 15% egg yolk displayed greater complete motility at 0, 3, and 6 hr postthaw and better progressive motility postthaw in contrast with sperm in Bioxcell (P < 0.05).
Sperm in Bioxcell displayed greater viability than sperm in each Tris-egg yolk extenders (P < 0.01), extra intact acrosomes than sperm in Tris-15% egg yolk (P < 0.05), and a bent for extra intact acrosomes than sperm in Tris-5% egg yolk (P < 0.10).
Sperm in Tris-5% egg yolk tended to have a better proportion of morphologically regular sperm in contrast with Bioxcell (P < 0.10). This research gives proof that markhor goats exhibit seasonality in scrotal circumference and testosterone ranges and that centrifugation could also be eradicated from the processing of markhor semen.